Cryopreservation of Cold-tender Apple Germplasm

Susceptibility of fruit from diverse apple and crabapple germplasm to attack from apple maggot (Diptera: Tephritidae).

Apple maggot, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), is a pest of major concern to apple, Malus x domestica (Borkh.) production in eastern North America. Host plant resistance to apple maggot among apple germplasm has been previously evaluated among a small number of exotic Malus accessions and domestic hybrid selections. However, a large number of exotic accessions housed in USDA...

III Vitro Germplasm Conservation of Atropa baetica by Cold Storage

Gerlllplaslll 01" ;/1 v;tro derived plantlels of Atroptl baeticfl was lIlainlained by cold storage al 4°C in darkness for six 1Il0nths. Tite response of Ihe cold stored gcrlllplasm to growing culture co nditions was sludied on Murasltige & Skoog mediullI supplelllented wilh BAP (0.66 mg r ), NAA (0.24 mg r\ inosilol (100 mg r ), Ihiamine (1 mg 1'1) :Hul 3% sucrose durillg a six month periodo Tl...

Genetic variation of germination cold tolerance in Japanese rice germplasm

Low temperatures at the initial stages of rice development prevent fast germination and seedling establishment and may cause significant productivity losses. In order to develop rice cultivars exhibiting cold tolerance, it is necessary to investigate genetic resources, providing basic knowledge to allow the introduction of genes involved in low temperature germination ability from accessions in...

Combining maize base germplasm for cold tolerance breeding

Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units.

Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies. In parallel, concerns are increasing about the technical and biological safety of these procedures. However, published data regarding the confirmed or theoretical hazards of these procedures are sparse a...